Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid
Hendrik Deschout , Raemdonck Koen, Stremersch S, Maoddi P, Mernier G, Renaud P, Jiguet S, Hendrix A, Bracke M, Van den Broecke R, Röding M, Rudemo M, Demeester J, De Smedt SC, Strubbe F, Neyts K, Braeckmans K.
Nanoscale, 2014, 6, 1741-1747, Date: 2014